Determination of Antibody Affinity Service

The binding force between an antibody and its antigenic determinant is called antibody affinity, and its essence is a non-covalent force. Antibody affinity embodies the binding ability of the antibody molecule for its antigenic determinant, and the determination of the affinity of an antibody for a specific antigen plays an important role in antibody screening. In general, the magnitude of the affinity can be characterized by either the binding constant Ka or the dissociation constant K_d.

Service Contents

Service Process	Customer Provides	Deliverables	Turnaround	Price
Monoclonal antibody production	Antigen: protein/polypeptide (requirements: 2-5 mg, 0.5 mg/ml, purity>90%) or Antigen: protein/polypeptide sequences	2-5 positive clones Antibody: 3-5 mg (ELISA> 1:128,000) Affinity determination report (including Kd, Ka) QC data	4~6 months	Inquiry
Determination of antibody affinity			1-2 weeks

Determination Method for Antibody Affinity

• Bio-layer interferometry (BLI)

The antibody affinity (K_D) was determined accurately with the use of BLI technique based on automatic detection platform (Fortebio Octet) .

Related Services

• Identification of Antibody Subtypes Service
• Antibody Pairing Service
• Antibody labeling service

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