Hybridoma Sequencing

Hybridoma sequencing is generally based on reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technologies. RACE is an effective method based on PCR, which rapidly amplifies the 5′ and 3′ ends of cDNA from a low number of transcripts, and is widely used because it is simple, fast and inexpensive.

Biologics International Corp. (BIC) has years of experience in hybridoma sequencing. First, we will extract your mRNA from your hybridoma cells and conduct RT-PCR to obtain the cDNA. Then, the light chain, heavy chain, or full-length fragment will be amplified by PCR or 3′ and 5′ RACE. The fragments can be connected to T-vectors and sequenced. The sequencing range covers mice, rats, rabbits, goats, chickens, and other species. BIC has a mature antibody preparation platform, and also provides a recombinant antibody service.

Service Content

Description	Customer Provides	Deliverables	Timeline	Price
Leader Sequence & Variable Domain Sequencing	Antigen information or hybridoma cells (>1 × 106 cells)	Detailed work performance report Sequence data Plasmid containing antibody gene sequence	3~4 weeks	Please inquire
Full-Length Antibody Sequencing

Why to Sequence a Hybridoma Antibody

• Articles and patents: antibody sequencing data are necessary to distinguish new antibodies from other antibodies.
• Antibody recombination: the antibody sequence is indispensable to produce high-quality, highly stable, and low-cost recombinant antibodies simply and rapidly.
• Antibody humanization: antibody sequence data are essential for antibody humanization, to rescue the antibody gene before hybridoma cell lines fail to grow or Ab production is lost.

What We Offer

• Professional technology: RT-PCR and RACE combined.
• Rigorous scientific research attitude: more than five positive clones will be sequenced to avoid pseudogenes interference.
• Various sequencing species: covering mice, rats, rabbits, goats, chickens, and other species.
• Comprehensive service content: providing hybridoma sequencing (genetic level) and De Novo sequencing (amino acid level).

Workflow

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