Custom Antibody Production Service

Biologics International Corp. (BIC) was founded by a group of skilled scientists who have specialized in antibody production for decades. After more than 3,000 successful projects, we are proud of ourselves in being able to provide high quality antibodies with affordable price. With the extensive experience in hybridoma, CHO, and HEK-293 cells, our custom antibody production service does not only offer you antibodies, but provides the complete support for all steps in antibody production.

Please remember our guarantee: Using protein antigen, ELISA titer of the antibody produced by BIC is greater than 1: 128,000. Feel free to contact us for any questions.

Why BIC?

• High ELISA titer guaranteed!
• Excellent antigen design.
• Rich selection of host species.
• Further antibody services are available, such as antibody labeling, sequencing, recombinant production, etc.

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Custom Antibody Production Categories

How to Select Antibodies

Do not know how to select above antibody types? Two factors should be taken into consideration before the start of each project design. One is the intended application, and the other is the choice of host species.

Monoclonal antibodies and polyclonal antibodies have their own advantages and disadvantages for different applications.

Projects	Pros	Cons	Species
Monoclonal Antibody	Unlimited quantity and availability of antibody Lot-to-lot consistency High specificity Quick reproduction of antibody once hybridoma is developed	Longer production time Requires a minimum of 4 mg immunogen Higher cost of development Antibodies less likely to work for multiple applications More difficult if using peptide immunogens Low affinity	Mouse Rabbit
Polyclonal Antibody	Quick turnaround Inexpensive High affinity One antibody can work for a variety of applications Requires less than 1mg immunogen Multiple host species, including non-mammalian hosts Better at detecting denatured proteins	High requirement for the purity of immunogen Requires a minimum of 4 mg immunogen Prone to produce non-specific antibodies, and produce background signals in some applications. Quantity available limited by the number of bleeds Lot-to-lot variability in characterization Difficulty to reproduce	Chicken Goat Rabbit Guinea Pig Mouse Rat Sheep

The following table provides advantages and disadvantages of each host species.

Species	Advantages	Disadvantages
Mouse	Common host species for antibody generation Small amount of antigen required Easy initial assays Low cost	Not suitable for short peptide antigens Small amount of serum
Rabbit	Good host species for antibody production High response to peptide and most protein antigens Broad epitope recognition Moderate cost	Not ideal for highly conserved mammalian proteins
Rat	Small amount of antigen required Ideal host for mouse antigen Easy initial assays Good for small-volume polyclonal antibody projects Low cost	Small amount of serum
Goat	Good for large volume polyclonal antibodies Large volume antibody production Large mammalian host species Easy initial assays	Not suitable for modified peptides Large antigen quantities High cost
Chicken	High specificity against antigen Suitable for polyclonal antibody production Good yield Moderate cost	Difficult to isolate antibodies Not ideal for the most peptide antigens

The Process of Antibody Production In BIC

Using classical protocols that have been proven most effective by ourselves, the antibody production in BIC can ensure the quality and save your precious time and money. The detailed process are followed:

Monoclonal and polyclonal antibody production has the similar process, except polyclonal antibody does not require cell fusion and screening. Antibody production generally starts from antigen analysis and design.

Antigen Design

With in-house antigen design tools, our scientists can rapidly and accurately analyze the protein sequence and predict which regions are immunogenic, unique and exposed. With years of experience in exploring protein field, BIC would analyze and design antigen, for producing high quality antibodies, even for those difficult ones.

Immunization

After antigen preparation, five balb/c mice or two New Zealand rabbits are immunized with multiple rounds of immunization and test-bled (ELISA test is employed). At this stage, BIC usually take at least four rounds of immunization, until the ELISA titer of the last test-bleed is greater than 1:50,000. Please be noted, the host of custom antibody has a wide selections, such as mouse, rabbit, rat, chicken, goat, etc.

Cell Fusion

According the ELISA result, spleen cells are harvested from two or three mice for hybridoma fusion.

Screening

For screening positive clones and long-term stability, parental cell lines can be subcloned through limiting-dilution cloning to obtain daughter cell lines.

Antibody Production and Purification

To monoclonal antibody, the antibodies are produced via cells cultured in roller bottles. To polyclonal antibody, the serum would be harvest for antibody purification after test-bleed. Antibody purification is affinity purification method using Protein A/G resin.

Detection and Labeling

Detection methods, such as Western blot, ELISA, antibody purification test, and endotoxin detection, would be employed. And we offer a wide variety of antibody labeling options for your subsequent experiments.

Applications

Our antibodies are backed by a 100% satisfaction guarantee to work in diverse applications, including Western blot, flow cytometry (FC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), as antibody-drug conjugates, and as ELISA detection antibodies (direct, indirect, sandwich, and capture ELISA formats).

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