Yeast Expression System

 Yeast expression systems allow efficient production of secreted and intracellular targets. In addition, yeast provides the advantage of post-translational modifications, which may be beneficial for some recombinant proteins. In BiologicsCorp, various host cells and vectors are available for yeast expression.We also have some robust secretion signal sequences to ensure the highest secretion of proteins.

• Codon optimization, gene synthesis, Subclone gene of interest into yeast expression vector
• Transforming Pichia pastoris, Saccharomyces cerevisiae or Kluyveromyces lactis
• Screening and Strain creation
• Expression evaluation and optimization
• Pilot-scale protein expression and purification
• Large-scale protein expression and purification (culture volume up to 500L)
• Process Development

Yeast system

Description	Deliverables		Time
Cat.: PS1102 Subcloning Linearization Transforming Expression optimization Expression verification Protein Purification Soluble protein with Tag QC by SDS-PAGE, Western blot, QC data	1-5mg	Purity>80%	6 weeks
		Purity>90%	7 weeks
Cat.: PS1108        Customized service for specific requirements Subcloning, Expression and Purification Tag-free, Endotoxin removal  (optional) QC by SDS-PAGE, Western Blot, MOLDI-TOF or MS	Customized Protein QC data Protocol in details

Related Services

• recombinant protein expression services
• bacterial expression system
• mammalian expression system
• yeast expression system

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