Recombinant Protein Production

Recombinant protein production, as one of the recombinant protein technologies, plays a critical role in academic research and biotech/pharmaceutical drug discoveries. Biologics International Corp (BIC) provides cell-based recombinant protein production services that can help you accelerate your discovery in a timely and cost-effective manner at an affordable price.

Service Highlights

• Guaranteed package! No-success no-fee for bacterial system.
• Risk Free. Till date, we have successfully delivered 3,000+ recombinant proteins with a very high success rate.
• Various types of proteins. Enzymes, cytokines, growth factors, hormones, receptors, transcription factors, antibodies, etc.
• Advance your project. From codon optimization, sequence analysis, to expression and purification, even stable cell line construction.
• Rich customizable selections. Four expression systems, fed-batch fermentation (1-100 L), protein-protein interaction analysis, protein tags, labeling, endotoxin levels, etc.

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Protein Expression Systems

Comparison of Protein Expression Systems

The selection of the system depends on the type of protein, the requirements for functional activity and the desired yield. These expression systems include mammalian, bacterial, yeast and insect.Several factors need to be taken into consideration, including target protein property, intended application, protein yield and cost. Furthermore, challenges exist for many protein expression projects, especially for large protein, membrane protein, nuclear protein, and proteins with heavy post-translational modifications. Each system has advantages and challenges, and choosing the right system is important for successful recombinant protein expression.

System	Advantages	Challenges	Applications
Mammalian	▪  Moderately rapid expression ▪  Higest-level protein processing ▪  Works well for secreted and    membrane proteins ▪  Can produce proteins either    transient, or by stable expression ▪  Can be used in static or    suspension culture	▪  Expensive ▪  Stable expression time ▪  Difficult to scale-up ▪  More demanding    culture condition ▪  Yields of intracellular    proteins are low	▪  Expression of    proteins complexed ▪  Functional assays ▪  Structural analysis ▪  Protein interactions ▪  Antibody production ▪  Virus production
Bacterial	▪  Rapid expression ▪  Less expensive ▪  Simple culture condition ▪  Simple process scale-up	▪  Protein solubility ▪  No eukaryotic post-    translational modifications ▪  Some proteins are not    properly folded ▪  Proteins are rarely secreted ▪  May be difficult to expression    some mammalian proteins	▪  Functional assays ▪  Structural analysis ▪  Protein interactions ▪  Antibody production
Insect	▪  similar to mammalian    protein processing ▪  Moderately rapid expression ▪  Works well for secreted,    membrane and intracellular    proteins ▪  Most protein folding and    post-translational modifi-    cations are possible	▪  Expensive ▪  Difficult to scale-up ▪  More demanding culture condi-    tion than prokaryotic systems ▪  Production of baculovirus    vectors is time consuming ▪  Characteristic N-linked glycan    structures of proteins are    different when compared to    the typical mammalian proteins	▪  Expression of    intraceliular proteins ▪  Functional assays ▪  Structural analysis ▪  Protein interactions
Yeast	▪  Eukaryotic protein processing ▪  Scalable up to fermentation    grams per liter ▪  Simple media requirements	▪  Fermentation required    for very high yields ▪  Growth condition may    require optimization	▪  Functional assays ▪  Structural analysis ▪  Protein interactions ▪  Antibody production

Protein Purification

Protein purification is vital for the characterization of the function, structure, and interactions of proteins. Separation of the protein of interest from all impurities, is typically the most challenging aspect of protein purification. Through the diversity of proteins and the customers with whom we have worked, our scientists have acquired the agility to customize downstream processes to each specific case. Insoluble proteins can also be purified and refolded to the native state. We routinely purify proteins using affinity chromatography, ion-exchange, size-exclusion, hydrophobic interaction and other techniques.

• Size-exclusion: separates proteins based on their differences in size.
• Affinity chromatography: takes advantage of the reversible interactions between a protein and its specific ligand linked to the chromatography matrix.
• Ion-exchange: exploits the reversible interactions between a protein and a charged chromatography medium.
• Hydrophobic interaction: exploits the reversible interactions between a protein and a hydrophobic ligand bound to the chromatography matrix.

Additional Services

• Protein or Antibody Labeling

Protein or antibody- labeling technique refers to the covalent attachment of molecules such as biotin, enzymes, and radioactive isotopes to specific sites of the target protein. BIC has developed a series of protein labeling techniques. The most popular labels that our customers choose include FITC, enzyme conjugates (horseradish peroxidase and alkaline phosphatase), and biotin.

• Endotoxin Removal

Endotoxins are major contaminants in commercially available proteins or biologically active substances. Endotoxin-contaminated protein samples transfected into cells or injected into an animal host can initiate a strong immune response such as endotoxin shock, tissue injury, and even death.
BIC understands that a reliable and accurate endotoxin test and removal of endotoxin from your biological sample are extremely important for downstream applications. We have extensive experience in endotoxin removal. We guarantee the effective reduction of endotoxin level to below 0.01-1 EU/µg or even less and meet the strict requirements for downstream animal and cell experiments.

• Protein Interaction Analysis

Protein-protein interaction plays a key role in predicting the protein function of the target protein and drug ability of molecules. Althoughseveral proteins perform their functions independently, the majority of proteins interact with others to execute a full range of biological activities.

In addition, BIC have ability to provide tag-free protein purification, RNAase removal and large scale protein production.

Why Work With Us?

As a biological CRO company, BIC has long devoted itself to the R&D of technologies related to recombinant protein and antibody. Our advanced protein expression optimization technology maximizes the yield and purity of the proteins to tailored to your needs. Our one-stop service covers every step from gene cloning to protein purification including downstream processing steps, such as protein desalting, lyophilization, vialing, and conjugation to tags, fluors, or other labels. We guarantee the delivery of proteins of desired quantity and purity. In addition to recombinant protein production services, we provide other protein services, including endotoxin removal, tag-removal, fermentation and additional quality assurance services.

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