High-throughput Protein Production Service

The emerging field of functional proteomics is driving the need to develop high-throughput technology to express and isolate a large number of proteins quickly for comprehensive analyses. Biologics International Corp (BIC) offers an innovative high-throughput protein production for bacterial protein expression system (typically several hundreds to thousands are involved), therefore to free up your time and speed up your projects.

Highlights of High-throughput Protein Production Service

• High throughput capability towards gene synthesis and expression
• One-step protein purification by Magnetic Agarose Beads
• Fast and cost-effective methods for delivering multiple purified proteins

High-throughput Protein Production Service Description

Project process		Descriptions	Timeline	Deliverables
	Multiple gene synthesis & subcloning in parallel (optional)	Codon optimization HTP gene synthesis Subcloning into E.coli expression vectors	<2 weeks	Sequencing chromatograms covering your genes (electronic) expression constructs 10-100 μg purified recombinant proteins (in 96-deep well plates) QC data of proteins (SDS-PAGE or dot blot)
	High-throughput protein expression	High-throughput protein expression in 96- or 24-deep-well block formats Detect expression level by SDS-PAGE or dot blot	<1 week
	High-throughput protein purification	Automated purification process on Magnetic Agarose Beads	<1 week

Additional services: Bacterial Expression System, Tag-free Protein, Fermentation Service, Endotoxin Removal

High-throughput Technology

• Affinity tags enable simultaneous purification for highly dissimilar proteins with the use of one standardized procedure, which is essential for high-throughput technologies.
• The development of plate-based purification strategies that allow the rapid capture of 24-96 different proteins and their subsequent purification by affinity resins has greatly expedited multi-parallel applications.
• One-step and automated purification process on Magnetic Agarose Beads has also been another major contribution for high-throughput protein production. The highly specific and irreversible binding to the beads enables efficient protein capture regardless of the expression level and eliminates protein loss during wash steps, resulting in high protein recovery.

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