Inducible Protein

Inducible Protein Most high yield E. coli protein production systems are with the target gene under an inducible promoter. The strain is first cultivated under healthy growth conditions in order to build up the cell mass. The culture is then subjected to an induction, whether it’s a physical event such as a temperature shift or […]

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Vaccine candidate for newly emerged H7N9 influenza virus

Vaccine candidate for newly emerged H7N9 influenza virus iBio, Inc. has produced a vaccine candidate for the newly emerged H7N9 influenza virus by an independent third party laboratory using the iBioLaunch platform. This validation milestone was achieved in 21 days as measured from initial antigen sequence information to purification of recombinant protein. The iBioLaunch platform […]

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A breakthrough in cancer treatment: GLUT1 crystal structure

A breakthrough in cancer treatment: GLUT1 crystal structure GLUT1 crystal structure please go to Read more in nature Introduction of GLUT1 transporter Glucose transporter 1 (or GLUT1) with highly conservatism, facilitates the transport of glucose across the plasma membranes of mammalian cells. read more in wikipedia GLUT1 Inhibitor WZB117 – A small-molecule inhibitor of glucose […]

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The Difficult of Eukaryotic

The Difficult Of Eukaryotic Genes Expression By Bacteria When bacteria are used to produce a eukaryotic protein, it is desirable to design the system so as to produce as large an amount of the protein as possible. There are several such systems for proteins expression in E.coli. For instance, One system uses phage T7 RNA […]

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Algae virus can jump to mammalian cells

Algae virus can jump to mammalian cells New research led by the University of Nebraska-Lincoln has provided the first direct evidence that an algae-infecting virus can invade and potentially replicate within some mammalian cells. Known as Acanthocystis turfacea chlorella virus 1, or ATCV-1, the pathogen is among a class of chloroviruses long believed to take […]

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Bacterial protein can help convert stem cells into neurons

Bacterial protein can help convert stem cells into neurons As the recipe book for turning stem cells into other types of cells keeps growing larger, the search for the perfect, therapeutically relevant blend of differentiation factors is revealing some interesting biology. A new study, for example, found that a protein in E. coli bacterial combined […]

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Alzheimer’s Disease breakthrough: Humam Gamma secretase structure

Alzheimer’s Disease breakthrough: Humam Gamma secretase structure The γ-secretase complex, comprising presenilin 1 (PS1), PEN-2, APH-1 and nicastrin, is a membrane-embedded protease that controls a number of important cellular functions through substrate cleavage. Aberrant cleavage of the amyloid precursor protein (APP) results in aggregation of amyloid-β, which accumulates in the brain and consequently causes Alzheimer’s […]

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IPTG Induction Protocol

IPTG Induction Protocol IPTG induction in bacteria can be performed using one of two basic methods. Fast induction does not work for all proteins and can give you suboptimal yields. Slow induction can enhance the solubility of some proteins. The method that is best for you will depend on your particular protein and the application. […]

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IPTG Induction Protein Expression

IPTG Induction Theory Isopropyl β-D-1-thiogalactopyranoside (IPTG, also known as lad-y) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce E.coli protein expression where the gene is under the control of the lac operator. Like […]

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