How to Increase Soluble Protein Expression

How to Increase Soluble Protein Expression How to improve protein solubility To answer this question, we should know what factors contribute the protein solubility. For a particular protein, the electrostatic charge plays an important role in its solubility. For different proteins, the protein production processes also affect the solubility of particular protein. The isoelectric points […]


Fusion Tags Enhance The Solubility Of Expressed Proteins

Fusion Tags Enhance The Solubility Of Expressed Proteins The soluble of heterologous proteins remains a serious bottleneck in protein production. Although alteration of expression conditions can sometimes solve the problem, however, a systematic analysis of the utility of these solubility fusions has been difficult, and it appears that many proteins react differently to the presence […]


Bacterial Endotoxin Definition

Bacterial Endotoxin Definition Lipopolysaccharides (LPS), also known as lipoglycans and endotoxins, Endotoxins are part of the outer membrane of the cell wall of Gram-negative bacteria. Although the term “endotoxin” is occasionally used to refer to any cell-associated bacterial toxin, in bacteriology it is properly reserved to refer to the lipopolysaccharide complex associated with the outer […]