Polyclonal Antibody Production Service

Polyclonal antibody (pAb) is obtained from different B-lymphocytes after reaction with corresponding antigens. Biologics Internation Corp (BIC) offers one-stop antibody production services and provides high quality pAb for qualitative immune-detection assays.

Key Features

Risk-free

No pAb delivery no payment.
Guaranteed ELISA titer (>1:128,000)
Positive WB results

Cutting edge technical strategies

In-house antigen design tools: high success rate
Soluble protein production and optimization: maximal native epitopes
Antigen affinity purification: improved antibody specificity

Comprehensive service packages

Start from gene synthesis to polyclonal antibody production
Multiple additional services to provide maximal flexibility in custom polyclonal antibody production services

Polyclonal Antibody Production Service Details

Cat. No.	PA1015	PA2005
Content	Full-EpitopeTM polyclonal antibody production service	Multi-pepTM polyclonal antibody production service
Antigen	Soluble protein 8-10 mg, >90% purity	Three conjugated peptide 1mg each, 85% purity
Key deliverables	2-5 mg antibody purified by antigen affinity purification 100 μg soluble antigen	Three antibody (2-5 mg each) purified by antigen affinity purification Three peptide (1 mg each)
ELISA titer	Guaranteed, >1:128,000	Guaranteed, >1:64,000
Western blot	Guaranteed positive results	NA
Animal	Two New Zealand rabbits (Please inquire for other animal species.)
Price	Inquire

Custom Polyclonal Antibody Production Process

Step 1: Antigen preparation

Recombinant protein production: codon optimization and soluble protein expression available for antigen production.
Peptide synthesis: Antigen design and conjugate to an immunogenic carrier protein for immunization.

Step 2: Immunization

Immunization and bleeding of two rabbits

Step 3: Custom antibody purification

Antigen affinity purification: obtain polyclonal antibody with high titer and specificity.

Selection Guide of Animal Species

Rabbit antibodies are considered as the first choice in most polyclonal antibody productions. Chicken are good substitute for rabbits when using mammalian antigens especially those that are highly conserved. If you are looking for large volume polyclonal antibody production, large animal such as goat or sheep can be used as host species to obtain enough polyclonal antibodies to meet your requirements.

	The most commonly used animal in pAb production Respond to broad antigens Moderate cost		Large volume antibody production Good yield High cost
	Low cost Small amount antibody production Mostly used for mAb production		High specificity against antigen Good yield Moderate cost