Shc1(Phospho-Tyr427) Polyclonal Antibody

Description	Rabbit Polyclonal
Synonyms	SH2 domain protein C1; SHC; SHC-transforming protein 1; SHCA; Src homology 2 domain-containing-transforming protein C1
Formulation	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Application	WB IHC IF
Application Details	Predicted MW: 46 52 kd Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Specificity	The antibody detects endogenous level of Shc1 only when phosphorylated at tyrosine 427.
Immunogen	Peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y(p)-V-N derived from Human Shc1.
Key Properties	Hu Ms Rt The antibody detects endogenous level of Shc1 only when phosphorylated at tyrosine 427. Peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y(p)-V-N derived from Human Shc1.
Purifiction	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Species Reactivity	Hu Ms Rt
Storage	Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

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