PTH 1-84 | Parathyroid Hormone 1-84, human
Parathyroid hormone (PTH) is the most important endocrine regulator of calcium and phosphorus concentration in extracellular fluids. This hormone is secreted from cells of the parathyroid glands and finds its major target cells in bone and kidney. Another hormone, parathyroid hormone-related protein, binds to the same receptor as parathyroid hormone and has major effects on development. Recombinant Human full length PTH 1-84 has potential as an anti-osteoporotic agent due to its properties as a bone formation stimulant, which increases bone turnover, stimulates osteoblasts and reduces both vertebral and non vertebral fractures.
Full Name | Parathyroid Hormone (1-84) Human Recombinant |
Synonyms | PTH 1-84,Parathyrin, PTH, Parathormone. |
Source | E. coli |
Amino Acid Sequence | SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ. |
Molecular Weight | Parathyroid Hormone 1-84 (full length) Human Recombinant was produced in E. coli as a single, non-glycosylated, polypeptide chain containing 84 amino acids and shown to have a MW of 10 kDa. |
Purity | >95% as determined by SDS-PAGE and HPLC |
Endotoxin | Endotoxin level was found to be ≤ 0.1 ng/µg(1EU/µg) by a LAL gel clot method. |
Formulation | Lyophilized from a 20 mM phosphate buffer, pH 7.0. |
Reconstitution | To reconstitute the protein, add sterile water to the lyophilized protein and make a preparation with a concentration ≥0.1mg/ml. The preparation can be diluted into other appropriate buffers if necessary. Cautions should be taken to avoid bacterial growth, resulting in potential contamination with endotoxin. |
Stability | Once lyophilized, the protein is stable at room temperature (≈3 weeks) and at -20°C (≥ 2 years from the date of receipt).Upon reconstitution, it can be stored at 4°C (≤ 7 days) or at -20°C (≤ 6 months). For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended.Avoid frequent freeze-thaw cycles. |
Biological Activity | The specific activity was determined by UMR106 cell/cAMP method to be 9,000 Units/mg. |
Usage | FOR LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes. |
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Product Code: CH113
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