PF 4 | Platelet Factor-4, Human

Full Name	Platelet Factor-4 Human Recombinant (CXCL4)
Synonyms	CXCL4, PF-4, PF4, Iroplact, Oncostatin-A, SCYB4, MGC138298.
Source	E. coli
Amino Acid Sequence	The sequence of the first four N-terminal amino acids was determined and was found to be Glu-Ala-Glu-Glu-Asp.
Molecular Weight	CXCL4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 70 amino acids and having a molecular mass of 7.8 kDa.
Purity	>95% as determined by SDS-PAGE and HPLC
Endotoxin	Endotoxin level was found to be ≤ 0.1 ng/µg(1EU/µg) by LAL gel clot method.
Formulation	Lyophilized from 50mM Tris-HCl pH 8.0 and 150mM NaCl buffer.
Reconstitution	To reconstitute the protein, add sterile water to the lyophilized protein and make a preparation with a concentration ≥0.1mg/ml. The preparation can be diluted into other appropriate buffers if necessary. Be cautious and avoid potential contamination with bacteria and endotoxin
Stability	The lyophilized form of the protein is stable at room temperature for 3 weeks and at -20°C for at least 2 years from the date of receipt. Upon reconstitution, the protein should be stored at 4°C for no more than 7 days. The protein is stable at -20°C for six months. For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended. Please prevent freeze-thaw cycles.
Biological Activity	The ED50 of CXCL4 as determined by its ability to inhibit human FGF basic dependent proliferation of NR6R3T3 mouse fibroblasts was found to be 5-15 µg/ml.
Usage	LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes.

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