NFkB-p65(Phospho-Thr254) Polyclonal Antibody

Description	Rabbit Polyclonal
Synonyms	NFKB3; RELA; TF65; Transcription factor p65; p65
Formulation	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Application	WB IHC IF
Application Details	Predicted MW: 65kd Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Specificity	The antibody detects endogenous level of NF-kB p65 only when phosphorylated at threonine 254.
Immunogen	Peptide sequence around phosphorylation site of threonine 254 (F-R-T(p)-P-P) derived from Human NFkB-p65.
Key Properties	Hu Ms Rt The antibody detects endogenous level of NF-kB p65 only when phosphorylated at threonine 254. Peptide sequence around phosphorylation site of threonine 254 (F-R-T(p)-P-P) derived from Human NFkB-p65.
Purifiction	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Species Reactivity	Hu Ms Rt
Storage	Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

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