Histone H2A.X(Phospho-Ser139) Polyclonal Antibody

Description	Rabbit Polyclonal
Synonyms	H2A.X; H2AFX; H2a/x; HIST5-2AX;
Formulation	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Application	WB IF
Application Details	Predicted MW: 15kd Western blotting: 1:500~1:1000 Immunofluorescence: 1:100~1:200
Specificity	The antibody detects endogenous level of Histone H2A.X only when phosphorylated at serine 139.
Immunogen	Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X.
Key Properties	Hu The antibody detects endogenous level of Histone H2A.X only when phosphorylated at serine 139. Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X.
Purifiction	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Species Reactivity	Hu
Storage	Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

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