HER2(Phospho-Tyr877) Polyclonal Antibody

Description	Rabbit Polyclonal
Synonyms	C-erbB-2; ErbB2;
Formulation	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Application	WB IHC IF
Application Details	Predicted MW: 185kd Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Specificity	The antibody detects endogenous level of HER2 only when phosphorylated at tyrosine 877.
Immunogen	Peptide sequence around phosphorylation site of tyrosine 877 (T-E-Y(p)-H-A) derived from Human HER2.
Key Properties	Hu Ms Rt The antibody detects endogenous level of HER2 only when phosphorylated at tyrosine 877. Peptide sequence around phosphorylation site of tyrosine 877 (T-E-Y(p)-H-A) derived from Human HER2.
Purifiction	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Species Reactivity	Hu Ms Rt
Storage	Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

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