BDNF Human

Full Name	Brain-Derived Neurotrophic Factor Human Recombinant
Synonyms	Brain-Derived Neurotrophic Factor, BDNF, MGC34632.
Source	Escherichia Coli.
Amino Acid Sequence	The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ser-Asp-Pro .
Molecular Weight	Recombinant Human Brain-derived neurotrophic factor produced in E. coli is a non-glycosylated polypeptide chain containing 119 amino acids.Bioactive BDNF is a non-covalently linked homodimer and having a molecular mass of 27kDa.
Purity	>95% as determined by SDS-PAGE and HPLC
Endotoxin	Endotoxin level was found to be ≤ 0.1 ng/µg(1EU/µg) by LAL gel clot method.
Formulation	recombinant BDNF is lyophilized from a 0.2µm filtered solution of 20mM PB,250mM NaCl, pH 7.2.
Reconstitution	To reconstitute the protein, add sterile water to the lyophilized protein and make a preparation with a concentration ≥0.1mg/ml. The preparation can be diluted into other appropriate buffers if necessary. Be cautious and avoid potential contamination with bacteria and endotoxin
Stability	The lyophilized form of the protein is stable at room temperature for 3 weeks and at -20°C for at least 2 years from the date of receipt. Upon reconstitution, the protein should be stored at 4°C for no more than 7 days. The protein is stable at -20°C for six months. For long term storage, please store at -80°C and the addition of a carrier protein (0.1% HSA or BSA) is recommended. Please prevent freeze-thaw cycles.
Biological Activity	The ED50 of rHuBDNF as determined by cell proliferation assay using the TrkB transfected BaF3 mouse pro-B cell line was 1-6ng/mL.
Usage	LABORATORY RESEARCH USE ONLY. Not intended for diagnostic or therapeutic purposes.

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