Akt2(Phospho-Ser474) Polyclonal Antibody

Description	Rabbit Polyclonal
Synonyms	PKB beta; Protein kinase B; RAC-PK-beta
Formulation	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Application	WB IHC
Application Details	Predicted MW: 60kd Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Specificity	The antibody detects endogenous level of Akt2 only when phosphorylated at serine 474.
Immunogen	Peptide sequence around phosphorylation site of serine 474 (Q-F-S(p)-Y-S) derived from Human Akt2.
Key Properties	Hu Ms Rt The antibody detects endogenous level of Akt2 only when phosphorylated at serine 474. Peptide sequence around phosphorylation site of serine 474 (Q-F-S(p)-Y-S) derived from Human Akt2.
Purifiction	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Species Reactivity	Hu Ms Rt
Storage	Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

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