AKT1(phospho-Thr450) Polyclonal Antibody

Description	Rabbit Polyclonal
Synonyms	RAC-PK-alpha; Protein kinase B;
Formulation	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Application	WB IHC IF
Application Details	Predicted MW: 60kd Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Specificity	The antibody detects endogenous level of AKT1 only when phosphorylated at threonine 450.
Immunogen	Peptide sequence around phosphorylation site of threonine 450 (T-I-T(p)-P-P) derived from Human AKT1.
Key Properties	Hu Ms Rt The antibody detects endogenous level of AKT1 only when phosphorylated at threonine 450. Peptide sequence around phosphorylation site of threonine 450 (T-I-T(p)-P-P) derived from Human AKT1.
Purifiction	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Species Reactivity	Hu Ms Rt
Storage	Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

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